collagen iv protein  (Elabscience Biotechnology)

Journal: Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie

Article Title: Ibrolipim attenuates early-stage nephropathy in diet-induced diabetic minipigs: Focus on oxidative stress and fibrogenesis.

doi: 10.1016/j.biopha.2020.110321

Figure Lengend Snippet: Fig. 4. Effects of Ibrolipim on the mRNA and protein expression of redox en- zymes. (A) Representative protein band images detected by Western blot. (B and F) The protein and mRNA expression of Nox-4. (C and G) The protein and mRNA expression of SOD1. (D and H) The protein and mRNA expression of CAT. (E and I) The protein and mRNA expression of GPX1. The relative protein and mRNA levels are normalized to internal control β-actin. Data are mean ± SD (n = 5 per group). ** P < 0.01 vs. CD group; # P < 0.05, ## P < 0.01 vs. HSFD group. CD, normal control diet; HSFD, high sucrose and fat diet; Nox-4, nicotinamide-adenine dinucleotide phosphate oxidase 4; SOD1, superoxide dismutase 1; CAT, catalase; GPX1, glutathione peroxidase 1.

Article Snippet: The sections were incubated with primary antibodies against Nox-4, SOD1, CAT, GPX1, collagen type IV alpha 1 (COL4A1), TGF-β1 or matrix metalloproteinase 2 (MMP-2) (1:100 rabbit polyclonal antibodies from Boster Biological Technology Co., China).

Techniques: Expressing, Western Blot, Control

Journal: Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie

Article Title: Ibrolipim attenuates early-stage nephropathy in diet-induced diabetic minipigs: Focus on oxidative stress and fibrogenesis.

doi: 10.1016/j.biopha.2020.110321

Figure Lengend Snippet: Fig. 5. Effects of Ibrolipim on the protein expression of redox enzymes detected by immunohistochemistry. (A) Representative immunohistochemical staining for Nox-4, SOD1, CAT and GPX1 (Original magnification ×400, Bar =20 μm). The expression of Nox-4 (B), SOD1 (C), CAT (D) and GPX1 (E) is quantitatively calculated as the percentage of positive area to total area (%). Data are mean ± SD (n = 5 per group). ** P < 0.01 vs. CD group; # P < 0.05, ## P < 0.01 vs. HSFD group. CD, normal control diet; HSFD, high sucrose and fat diet; Nox-4, nicotinamide-adenine dinucleotide phosphate oxidase 4; SOD1, superoxide dismutase 1; CAT, catalase; GPX1, glutathione peroxidase 1.

Article Snippet: The sections were incubated with primary antibodies against Nox-4, SOD1, CAT, GPX1, collagen type IV alpha 1 (COL4A1), TGF-β1 or matrix metalloproteinase 2 (MMP-2) (1:100 rabbit polyclonal antibodies from Boster Biological Technology Co., China).

Techniques: Expressing, Immunohistochemistry, Immunohistochemical staining, Staining, Control

Journal: Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie

Article Title: Ibrolipim attenuates early-stage nephropathy in diet-induced diabetic minipigs: Focus on oxidative stress and fibrogenesis.

doi: 10.1016/j.biopha.2020.110321

Figure Lengend Snippet: Fig. 8. Proposed mechanisms of HSFD-induced renal injury and the protective effects of Ibrolipim. The black arrows represent the de- teriorative pathogenesis of nutrient stress, and the red arrows and cross indicate the re- noprotective effects of Ibrolipim. Two-way ar- rows mean correlation, and the upward arrows imply activation while the downward arrows imply inhibition. ROS, reactive oxygen species; MDA, malondialdehyde; GSH, reduced glu- tathione; T-AOC, total antioxidant capacity; ECM, extracellular matrix; Nox-4, nicotina- mide-adenine dinucleotide phosphate oxidase 4; SOD1, superoxide dismutase 1; CAT, cata- lase; GPX1, glutathione peroxidase 1; TGF-β1, transforming growth factor-β1; MMP-2, matrix metalloproteinase 2 (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article).

Article Snippet: The sections were incubated with primary antibodies against Nox-4, SOD1, CAT, GPX1, collagen type IV alpha 1 (COL4A1), TGF-β1 or matrix metalloproteinase 2 (MMP-2) (1:100 rabbit polyclonal antibodies from Boster Biological Technology Co., China).

Techniques: Activation Assay, Inhibition